Ex situ konzervácia druhov ihličnatých drevín použitím in vitro kultúr a kryokonzervačných techník – v rámci projektu Integrovaný prístup konzervácie ohrozených rastlín pre 21. storočie
Ex situ conservation of conifer tree species using in vitro cultures and cryopreservation techniques – in the frame of An integrated approach to conservation of threatened plants for the 21st Century
Program: COST
Zodpovedný riešiteľ: RNDr. Salaj Terézia DrSc.
Annotation: The overarching aim of this Action is to improve plant conservation in Europe through the establishment of a network of scientists and other stakeholders who deal with different aspects of plant conservation, from plant taxonomy, ecology, conservation genetics, conservation physiology and reproductive biology to protected area\’s managers.The aim of research group at IPGB PSBC SAS is the study of somatic embryogenesis, including initiation of the process, maturation as well as plantlet regeneration in Pinus nigra, Abies alba and Abies hybrids. The study will be focused on physiological, biochemical as well as structural aspects, important for understanding of the mechanism underlying somatic embryogenesis. For cryopreservation of embryogenic tissue the slow-freezing method, suitable for unorganised cell and tissue cultures, will be used.
Project web page:
Duration: 15.10.2019 – 14.10.2023


Štúdium vplyvu sekretómu na zvýšenie embryogénnej kapacity pri vybraných druhoch ihličnanov
The study of secretome influence to increasing embryogenic capacity of selected Conifer species
Program: VEGA
Zodpovedný riešiteľ: Mgr. Klubicová Katarína PhD.
Annotation: Somatic embryogenesis represents an efficient plant regeneration system for theoretical studies as well as for mass propagation, especially in conifers. Although the process has been initiated for many conifer species, maturation of somatic embryos and somatic seedling regeneration needs deeper understanding. Advances in understanding of maturation and complete plant regeneration are necessary to use the regeneration system for conifer micropropagation. The aim of the submitted proposal is isolation and identification of a secretome of embryogenic cell lines with different embryogenic capacity using proteomic methods and to find potential markers or proteins active in regulation or signal transduction in selected conifer species and contribute to better understanding of process of somatic embryogenesis in woody plants.
Duration: 1.1.2018 – 31.12.2020
Štúdium vybraných fyziologických a molekulárnych parametrov zahrnutých v regenerácii a raste parazitických burín Phelipanche spp.
Study of selected physiological and molecular factors involved in regeneration and growth of parasitic weeds of Phelipanche spp.
Program: VEGA
Zodpovedný riešiteľ: RNDr. Matúšová Radoslava PhD.
Annotation: Parasitic plants of Phelipanche, Orobanche and Striga spp. belong to economically important plant pathogens with negative impact on agriculture. They are causing an annual loss of more then 7 billion US$. After germination these parasitic plants attach to the root system of host plants and acquire water and all nutrients from the host. There is no effective method to eliminate these weeds at present. Therefore the scientists focus their research on identification of genes and mechanisms involved in interactions between parasitic plants and their hosts. The main goal of this project is study of factors influencing growth and regeneration of Phelipanche spp. under in vitro conditions. This knowledge together with developed protocols for Phelipanche genetic transformation will allow to study functions of genes involved in parasite-host interactions and help in development of novel methods for the selective control of root parasitic weeds.
Duration: 1.1.2017 – 31.12.2020
MASnPNO – Využitie myrozinázy na aktiváciu sulforafanu pre vývoj preparátu s preventívnymi účinkami nádorových ochorení
The application of myrosinase for sulforaphane activation in development of a novel product exhibiting cancer prevention effects
Program: APVV
Zodpovedný riešiteľ: doc. RNDr. Salaj Ján DrSc.
Annotation: Effects of sulphoraphane on cencer prevention was already proven. Sulforaphane is often presents in food at the non-effective level for fulfillment the prevention effects. In addition, the natural glucosinolate form of sulforaphane, glucorafanine, is much less effective, therefore its activation to sulforaphane is needed. This conversion is catalysed by enzyme, myrosinase (EC Aim of the current project is to design the new product based on sulforaphane-glucosinolate and stabilized myrosinase as combine product.
Duration: 1.7.2017 – 30.6.2021