Hľadanie tolerantných odrôd – fenotypovanie na úrovni rastliny a bunky
The quest for tolerant varieties – Phenotyping at plant and cellular level
Program: COST
Zodpovedný riešiteľ: RNDr. Salaj Terézia DrSc.
Annotation: The project is focused on the study of physiological, biochemical and structural aspects of somatic embryogenesis. Somatic embryogenesis is development of embryos from somatic cells of a plant body under in vitro conditions. The process involves several steps as initiation of embryogenic tissues, maturation and germination of somatic embryos and transfer to soil. Each of the mentioned steps will be studied in details with aim to improve the process. The role of nutrient medium composition and explants will be tested for the initiation.The structure of somatic embryos will be followed by light microscopy.For maintenance of embryogenic tissues solid or liquid media will be used as well as for their long-term cultivation the method of cryopreservation will be tested. The maturation of somatic embryos is a very important step resulting in the whole plant (somatic seedlings) regeneration. The project focuses also on the optimization of maturation process and plantlet regeneration. An important part of the project is the study of embryogenic tissues and comparison to nonembryogenic callus culture by analysis using proteomic methods to get deeper insight to the process of somatic embryogenesis.
Duration: 22.5.2014 – 21.5.2018
Somatická embryogenéza vybraných druhov ihličnatých drevín: indukcia embryogénnych pletív, ich udržiavanie, maturácia somatických embryí a regenerácia rastlín
Somatic embryogenesis in selected conifer species: initiation of embryogenic tissues, their maintenance, maturation of somatic embryos and plantlet regeneration
Program: Medziakademická dohoda (MAD)
Zodpovedný riešiteľ: RNDr. Salaj Terézia DrSc.
Annotation: Somatic embryogenesis (SE) is an in vitro process of embryo differentiation from somatic cells of a plant body. In conifers the process involves several steps as: initiation of embryogenic tissues, their maintenance, somatic embryo maturation and plantlet (somatic seedlings) regeneration. Each step requires specific conditions and is affected by several factors. The submitted proposal intends to study the individual steps of SE, mostly the effect of primary explant developmental stage, plant growth regulators influence as well as osmotic potential of the culture medium. Species as Abies alba, Pinus sylvestris and Pinus nigra will be included as experimental material.
Duration: 1.1.2016 – 31.12.2018


Mäsožravé rastliny – perspektívny zdroj génov pre hydrolytické enzýmy s antifungálnym potenciálom
Carnivorous plants – prospective source of genes for hydrolytic enzymes with antifungal potential
Program: VEGA
Zodpovedný riešiteľ: Ing. Libantová Jana CSc.
Annotation: Chitinases play a role in the development and physiology of plants and in stress responses. The model object of this project is a carnivorous plant, Drosera rotundifolia L., from which the gene for chitinase (DrChit) involved in digestive processes of prey was isolated. As chitinous cell wall of fungal pathogens is also degraded by chitinases that are expressed during the plant defense, the DrChit has a potential use in biotechnology aimed at the plant resistance reinforcement. Here we focus on the isolation and purification of DrChit protein, its biochemical characterization and antifungal potential determination. Next, we will isolate and characterize the other genes with the carnivorous expression profile. To clarify the role of chitinases in defense, the chitinase expression profiles in embryogenic cultures of conifers exposed to abiotic stress will be evaluated as well. We suppose these results may contribute to the development of strategies for plant resistance to stress by genetic engineering approach.
Duration: 1.1.2017 – 31.12.2019
Štúdium vybraných fyziologických a molekulárnych parametrov zahrnutých v regenerácii a raste parazitických burín Phelipanche spp.
Study of selected physiological and molecular factors involved in regeneration and growth of parasitic weeds of Phelipanche spp.
Program: VEGA
Zodpovedný riešiteľ: RNDr. Matúšová Radoslava PhD.
Annotation: Parasitic plants of Phelipanche, Orobanche and Striga spp. belong to economically important plant pathogens with negative impact on agriculture. They are causing an annual loss of more then 7 billion US$. After germination these parasitic plants attach to the root system of host plants and acquire water and all nutrients from the host. There is no effective method to eliminate these weeds at present. Therefore the scientists focus their research on identification of genes and mechanisms involved in interactions between parasitic plants and their hosts. The main goal of this project is study of factors influencing growth and regeneration of Phelipanche spp. under in vitro conditions. This knowledge together with developed protocols for Phelipanche genetic transformation will allow to study functions of genes involved in parasite-host interactions and help in development of novel methods for the selective control of root parasitic weeds.
Duration: 1.1.2017 – 31.12.2020
MASnPNO – Využitie myrozinázy na aktiváciu sulforafanu pre vývoj preparátu s preventívnymi účinkami nádorových ochorení
The application of myrosinase for sulforaphane activation in development of a novel product exhibiting cancer prevention effects
Program: APVV
Zodpovedný riešiteľ: doc. RNDr. Salaj Ján DrSc.
Annotation: Effects of sulphoraphane on cencer prevention was already proven. Sulforaphane is often presents in food at the non-effective level for fulfillment the prevention effects. In addition, the natural glucosinolate form of sulforaphane, glucorafanine, is much less effective, therefore its activation to sulforaphane is needed. This conversion is catalysed by enzyme, myrosinase (EC Aim of the current project is to design the new product based on sulforaphane-glucosinolate and stabilized myrosinase as combine product.
Duration: 1.7.2017 – 30.6.2021