iPLANTA – Modifikácia rastlín na produkciu interferujúcej RNA
Modifying plants to produce interfering RNA
Program: COST
Zodpovedný riešiteľ: doc. Ing. Moravčíková Jana PhD.
Annotation: Modern research has identified the high potential of carnivorous plants for biotechnological use. However, regulatory mechanisms in biological and metabolic processes in these plants have not yet been elucidated. Different types of small RNA have an important role in these processes. These molecules regulate various aspects of plant developmental processes and are also involved in regulating the response of plants to environmental stress. The project focuses on the isolation and analysis of these molecules in Drosera plants. Insulating kits and available protocols will be the starting point, but the optimization of procedures will be necessary for the problem of these plants\’ knits. The obtained protocols for the isolation and analysis of small RNAs are a prerequisite for research into the regulation of individual processes in carnivorous plants such as prey digestion or nutrient recovery, environmental stress responses.
Duration: 27.10.2016 – 26.10.2020
Stresom-indukovaná expresia arabinogalaktanov a obranné proteíny v súvislosti s iniciáciou androgenézy
Stress-inducible expression of arabinogalactans and defence-related proteins in relation to androgenesis initiation
Program: Medziakademická dohoda (MAD)
Zodpovedný riešiteľ: doc. Ing. Moravčíková Jana PhD.
Annotation: Understanding of mechanisms leading to plant embryogenesis under inducible stress conditions (e.g. temperature and/or osmotic stress) at the molecular level is crucial in crop improvement. To overcome resistance of genotypes to microspore embryogenesis (ME) under in vitro conditions, selectable markers for responsiveness/recalcitrance are essential to be identified. Pathogenesis-related proteins (PRs, PR3; chitinases) involved in the plant defence (a)biotic responses with arabinogalactans (AGPs), probably functioned in cell division, differentiation and development, seem to be good candidates. The aim of this project is to characterize the expression of PR3 and AGPs in relation to ME induction effectiveness in rye (Secale cereale L.).
Duration: 1.1.2016 – 31.12.2018


Štúdium funkcie génov dehydrínov z Arabidopsis thaliana pri tolerancii voči vybraným typom abiotického stresu
Studying of the function of dehydrin genes from Arabidopsis thaliana in the tolerance to selected types of abiotic stresses
Program: VEGA
Zodpovedný riešiteľ: doc. Ing. Moravčíková Jana PhD.
Annotation: Due to the changes in the environment, plants are increasingly exposed to different types of abiotic stresses such as drought, salinity, low temperature or the presence of heavy metals. A part of plant response is a modulation of the cellular metabolism and production of a wide range of proteins including dehydrins. Dehydrins are hydrophilic proteins that are induced mainly under of low temperature or dehydration. The mechanism of their action has not been clarified yet. The work is aimed to study the effect of constitutive expression of Arabidopsis dehydrin genesAt1g54410, At2g21490 and At3g50970 on the tolerance of transgenic tobacco plants against selected types of abiotic stresses, and their combinations. Their structures contain histidine-rich regions that have potential to bind free metals; therefore, it could contribute to increased tolerance to heavy metals. At the same time we will study the extent to which the overexpression of dehydrin genes interact with other components of plant defense.
Duration: 1.1.2017 – 31.12.2019
PLADEFNUT – Štúdium vplyvu rôznych nutričných podmienok na akumuláciu toxických elementov v pšenici
Studying the effect of different nutritional conditions on the accumulation of toxic elements in wheat
Program: APVV
Zodpovedný riešiteľ: doc. Ing. Moravčíková Jana PhD.
Annotation: The project addresses the impact of two important environmental contaminants on plants at present,namely the presence of toxic metals, excessive amounts / concentrations of nitrogen and their combinations.Both factors are the result of the development of industry, agriculture and chemical production that areassociated with a large number of pollutants, primarily sulfur oxides, nitrogen, carbon emissions and heavymetals. Knowledge about plant defense mechanisms against these factors is limited. Especially the effects ofexcessive fertilization on accumulation of metals as well as on the plants themselves are at deeper levels littlestudied. The project will address this issue comprehensively through the study of antioxidant enzymes andquantitative/qualitative changes in profiles of typical plant defense proteins. In parallel line of project we willcharacterize a set of varieties of wheat grown in Slovakia for genetic potential to accumulate cadmium usingavailable molecular markers. The project will generate new knowledge about the molecular background of plantresponses to relatively little studied types of stress. These will be evaluated for potential use as markers fortolerance /sensitivity to metal toxicity or excessive application of fertilizers. Knowledge on the effect of increasingconcentrations of nitrogen to plants and their defense capacity will be useful for the management of fertilizerapplication in agriculture. The project will also contribute to the characterization of wheat germplasm fromSlovakia in terms of potential health risk when grown on contaminated territories.
Duration: 1.7.2016 – 30.6.2020