Mikropropagácia a kryoprezervácia menej známych druhov drobného ovocia
Micropropagation and cryopreservation of less known small fruit species
Program: VEGA
Zodpovedný riešiteľ: RNDr. Gajdošová Alena CSc.
Annotation: Berry fruits are characterized by high nutritional value and are considered as nutraceutical food. In spite of the increasing demand for berry fruits on the market their cultivation is not spread in Slovakia because of the lack of planting material of wider cultivar assortment and its high price. Rapid and profitable clonal propagation of planting material is possible to reach using tissue cultures. The project will be focused i) on elaboration of systems for micropropagation of some introduced less known small fruit species (Vaccinium, Rubus, Lonicera, Amelanchier spp.) utilizable in large-scale propagation of planting material; ii) on development of protocol for long-term storage of small fruit genetic resources by cryopreservation; iii) on testing of genetic fidelity of in vitro derived and after cryopreservation regenerated plants using molecular markers, what enables to evaluate suitability of elaborated protocols for their application for routine propagation and long-term storage of genetic resources.
Duration: 1.1.2017 – 31.12.2020
Molekulárne metódy v šľachtení prirodzene bezlepkového amarantu
Molecular methods in breeding of naturally gluten free amaranth
Program: VEGA
Zodpovedný riešiteľ: Ing. Hricová Andrea PhD.
Annotation: The efficient and high-throughput methods of current biology significantly reduce plant breeding process andallow to very precisely define valuable original plant material that can be obtained also by using modernbiotechnology approaches. These tools are great value to support conventional breeding for sustainable foodproduction especially under the climate change and meet demand over growing human population. By couplingthese modern approaches we will contribute to knowledge on genome and proteome of amaranth (Amaranth sp.),naturally gluten-free pseudocereal with exceptional nutrition attributes, that is becoming more recognized andappreciated in a diet. Main purpose of the project will be extensive characterization of first Slovak amaranthvariety PRIBINA as well as the other mutants previously generated by mutation breeding. Based on positivelychanged traits we will select another mutant lines, insert them to variety evaluation process to be registered asnew varieties.
Duration: 1.1.2016 – 31.12.2018
MASnPNO – Využitie myrozinázy na aktiváciu sulforafanu pre vývoj preparátu s preventívnymi účinkami nádorových ochorení
The application of myrosinase for sulforaphane activation in development of a novel product exhibiting cancer prevention effects
Program: APVV
Zodpovedný riešiteľ: doc. RNDr. Salaj Ján DrSc.
Annotation: Effects of sulphoraphane on cencer prevention was already proven. Sulforaphane is often presents in food at the non-effective level for fulfillment the prevention effects. In addition, the natural glucosinolate form of sulforaphane, glucorafanine, is much less effective, therefore its activation to sulforaphane is needed. This conversion is catalysed by enzyme, myrosinase (EC Aim of the current project is to design the new product based on sulforaphane-glucosinolate and stabilized myrosinase as combine product.
Duration: 1.7.2017 – 30.6.2021