Hľadanie tolerantných odrôd – fenotypovanie na úrovni rastliny a bunky
The quest for tolerant varieties – Phenotyping at plant and cellular level
Program: COST
Zodpovedný riešiteľ: RNDr. Salaj Terézia DrSc.
Annotation: The project is focused on the study of physiological, biochemical and structural aspects of somatic embryogenesis. Somatic embryogenesis is development of embryos from somatic cells of a plant body under in vitro conditions. The process involves several steps as initiation of embryogenic tissues, maturation and germination of somatic embryos and transfer to soil. Each of the mentioned steps will be studied in details with aim to improve the process. The role of nutrient medium composition and explants will be tested for the initiation.The structure of somatic embryos will be followed by light microscopy.For maintenance of embryogenic tissues solid or liquid media will be used as well as for their long-term cultivation the method of cryopreservation will be tested. The maturation of somatic embryos is a very important step resulting in the whole plant (somatic seedlings) regeneration. The project focuses also on the optimization of maturation process and plantlet regeneration. An important part of the project is the study of embryogenic tissues and comparison to nonembryogenic callus culture by analysis using proteomic methods to get deeper insight to the process of somatic embryogenesis.
Duration: 22.5.2014 – 21.5.2018
Somatická embryogenéza vybraných druhov ihličnatých drevín: indukcia embryogénnych pletív, ich udržiavanie, maturácia somatických embryí a regenerácia rastlín
Somatic embryogenesis in selected conifer species: initiation of embryogenic tissues, their maintenance, maturation of somatic embryos and plantlet regeneration
Program: Medziakademická dohoda (MAD)
Zodpovedný riešiteľ: RNDr. Salaj Terézia DrSc.
Annotation: Somatic embryogenesis (SE) is an in vitro process of embryo differentiation from somatic cells of a plant body. In conifers the process involves several steps as: initiation of embryogenic tissues, their maintenance, somatic embryo maturation and plantlet (somatic seedlings) regeneration. Each step requires specific conditions and is affected by several factors. The submitted proposal intends to study the individual steps of SE, mostly the effect of primary explant developmental stage, plant growth regulators influence as well as osmotic potential of the culture medium. Species as Abies alba, Pinus sylvestris and Pinus nigra will be included as experimental material.
Duration: 1.1.2016 – 31.12.2018


Molekulárne metódy v šľachtení prirodzene bezlepkového amarantu
Molecular methods in breeding of naturally gluten free amaranth
Program: VEGA
Zodpovedný riešiteľ: Ing. Hricová Andrea PhD.
Annotation: The efficient and high-throughput methods of current biology significantly reduce plant breeding process andallow to very precisely define valuable original plant material that can be obtained also by using modernbiotechnology approaches. These tools are great value to support conventional breeding for sustainable foodproduction especially under the climate change and meet demand over growing human population. By couplingthese modern approaches we will contribute to knowledge on genome and proteome of amaranth (Amaranth sp.),naturally gluten-free pseudocereal with exceptional nutrition attributes, that is becoming more recognized andappreciated in a diet. Main purpose of the project will be extensive characterization of first Slovak amaranthvariety PRIBINA as well as the other mutants previously generated by mutation breeding. Based on positivelychanged traits we will select another mutant lines, insert them to variety evaluation process to be registered asnew varieties.
Duration: 1.1.2016 – 31.12.2018
Štúdium vybraných fyziologických a molekulárnych parametrov zahrnutých v regenerácii a raste parazitických burín Phelipanche spp.
Study of selected physiological and molecular factors involved in regeneration and growth of parasitic weeds of Phelipanche spp.
Program: VEGA
Zodpovedný riešiteľ: RNDr. Matúšová Radoslava PhD.
Annotation: Parasitic plants of Phelipanche, Orobanche and Striga spp. belong to economically important plant pathogens with negative impact on agriculture. They are causing an annual loss of more then 7 billion US$. After germination these parasitic plants attach to the root system of host plants and acquire water and all nutrients from the host. There is no effective method to eliminate these weeds at present. Therefore the scientists focus their research on identification of genes and mechanisms involved in interactions between parasitic plants and their hosts. The main goal of this project is study of factors influencing growth and regeneration of Phelipanche spp. under in vitro conditions. This knowledge together with developed protocols for Phelipanche genetic transformation will allow to study functions of genes involved in parasite-host interactions and help in development of novel methods for the selective control of root parasitic weeds.
Duration: 1.1.2017 – 31.12.2020