ROLOFSYTS – Funkčná analýza synaptotagmínov so zreteľom na odpovede rastlín na environmentálne stresy
Functional analysis of synaptotagmins in responses of plants to environmental stresses
Program: APVV
Zodpovedný riešiteľ: Doc. RNDr. Jásik Ján DrSc.
Annotation: Crop yields are largely dependent on their tolerances to environmental influences, including salinity. Previously, we have shown that the Arabidopsis synaptotagmin 1 (AtSYT1), the most abundantly expressed Arabidopsis ortholog of the well-known calcium sensors in animal neurons, is involved in responses to abiotic stresses. The general goal of this proposal is to reveal the mechanisms by which AtSYTs regulate the reactions to high salinity and other stresses. We wish to characterize AtSYTs expression, describe phenotype of Atsyts mutant lines, to study subcellular localisation of AtSYTs, and to monitor intracellular dynamics of AtSYT1. Additionally, we shall address the functional analysis of the interaction fished out in the yeast two hybrid assay connecting AtSYT1 to the MAPK cascade. Our study will contribute to the overall understanding of how membrane trafficking is involved in stress responses.
Duration: 1.6.2017 – 30.6.2021
PlantCalp – Mechanizmus pozičnej signalizácie v rastlinách – pochopenie DEK1 dráhy
The mechanism of positional signalling in plants – understanding of the DEK1 pathway
Program: APVV
Zodpovedný riešiteľ: Doc. RNDr. Jásik Ján DrSc.
Annotation: Positional signalling plays instrumental role during plant growth and development. Positional cues control what type of cell will form (cell fate determination) and how big the cell will grow (cell expansion). Genetic studies identified membrane-anchored calpain protease DEFECTIVE KERNEL 1 (DEK1) as an essential regulator of plant growth and development. Our hypothesis is that DEK1 evolved both sensory and effector functions to control robust morphogenetic outputs such as cell expansion, asymmetric cell division and cell fate determination in response to local positional cues. The exact mechanism of DEK1 activation and signal transmission is unknown. Another crucial missing piece in our understanding of DEK1-mediated signaling is the identity of DEK1 molecular targets. In this project we aim to validate putative DEK1 substrate(s) and interacting partners, functionally characterize the role of phosphorylation in DEK1 activation and continue our efforts to solve the DEK1 3D structure. The project builds on published and recent unpublished work of the principal investigator and his collaborators. Unraveling the mechanism of DEK1 action will shed light on one of the key principles of developmental control in plants with broad implications for biotechnology and agriculture.
Duration: 1.8.2018 – 30.6.2022
Transkripčná, posttranskripčná a posttranslačná regulácia génu IRT1, rozhodujúceho transportéra železa v koreni rastlín
Transcriptional, post-transcriptional and post-translational regulation of IRT1 gene, a key transporter of iron in plant roots
Program: VEGA
Zodpovedný riešiteľ: Doc. RNDr. Jásik Ján DrSc.
Annotation: Using transgenic and mutant lines in combination with the current genetic, molecular biological, histochemical, immunological, fluorometric, chemical analytical and microscopic methods we want to explore whether and how the presence or absence of iron and other metal elements transferred by IRT1 protein may affect the activity of IRT1 promoter, the process of RNA splicing, localization, turnover and intracellular dynamics of IRT1 protein varieties in roots of the model organism Arabidopsis thaliana. Examination of multi-level regulation of the IRT1 gene and simultaneous monitoring of the contents and the distribution of iron and other metallic components in the plants will yield valuable information for applications in biotechnology in order to enrich the plants with iron and / or to limit the absorption of potentially toxic metal elements. The findings may be useful in the agricultural breeding practice to obtain higher yielding varieties and tolerant plants to environmental stress caused by heavy metals.
Duration: 1.1.2017 – 31.12.2020