Štúdium vplyvu sekretómu na zvýšenie embryogénnej kapacity pri vybraných druhoch ihličnanov
The study of secretome influence to increasing embryogenic capacity of selected Conifer species
Program: VEGA
Zodpovedný riešiteľ: Mgr. Klubicová Katarína PhD.
Annotation: Somatic embryogenesis represents an efficient plant regeneration system for theoretical studies as well as for mass propagation, especially in conifers. Although the process has been initiated for many conifer species, maturation of somatic embryos and somatic seedling regeneration needs deeper understanding. Advances in understanding of maturation and complete plant regeneration are necessary to use the regeneration system for conifer micropropagation. The aim of the submitted proposal is isolation and identification of a secretome of embryogenic cell lines with different embryogenic capacity using proteomic methods and to find potential markers or proteins active in regulation or signal transduction in selected conifer species and contribute to better understanding of process of somatic embryogenesis in woody plants.
Duration: 1.1.2018 – 31.12.2020
Štúdium vybraných fyziologických a molekulárnych parametrov zahrnutých v regenerácii a raste parazitických burín Phelipanche spp.
Study of selected physiological and molecular factors involved in regeneration and growth of parasitic weeds of Phelipanche spp.
Program: VEGA
Zodpovedný riešiteľ: RNDr. Matúšová Radoslava PhD.
Annotation: Parasitic plants of Phelipanche, Orobanche and Striga spp. belong to economically important plant pathogens with negative impact on agriculture. They are causing an annual loss of more then 7 billion US$. After germination these parasitic plants attach to the root system of host plants and acquire water and all nutrients from the host. There is no effective method to eliminate these weeds at present. Therefore the scientists focus their research on identification of genes and mechanisms involved in interactions between parasitic plants and their hosts. The main goal of this project is study of factors influencing growth and regeneration of Phelipanche spp. under in vitro conditions. This knowledge together with developed protocols for Phelipanche genetic transformation will allow to study functions of genes involved in parasite-host interactions and help in development of novel methods for the selective control of root parasitic weeds.
Duration: 1.1.2017 – 31.12.2020