Mäsožravé rastliny – perspektívny zdroj génov pre hydrolytické enzýmy s antifungálnym potenciálom
Carnivorous plants – prospective source of genes for hydrolytic enzymes with antifungal potential
Program: VEGA
Zodpovedný riešiteľ: Ing. Libantová Jana CSc.
Annotation: Chitinases play a role in the development and physiology of plants and in stress responses. The model object of this project is a carnivorous plant, Drosera rotundifolia L., from which the gene for chitinase (DrChit) involved in digestive processes of prey was isolated. As chitinous cell wall of fungal pathogens is also degraded by chitinases that are expressed during the plant defense, the DrChit has a potential use in biotechnology aimed at the plant resistance reinforcement. Here we focus on the isolation and purification of DrChit protein, its biochemical characterization and antifungal potential determination. Next, we will isolate and characterize the other genes with the carnivorous expression profile. To clarify the role of chitinases in defense, the chitinase expression profiles in embryogenic cultures of conifers exposed to abiotic stress will be evaluated as well. We suppose these results may contribute to the development of strategies for plant resistance to stress by genetic engineering approach.
Duration: 1.1.2017 – 31.12.2019