PlantCalp – Mechanizmus pozičnej signalizácie v rastlinách – pochopenie DEK1 dráhy
The mechanism of positional signalling in plants – understanding of the DEK1 pathway
Program: APVV
Zodpovedný riešiteľ: Doc. RNDr. Jásik Ján DrSc.
Annotation: Positional signalling plays instrumental role during plant growth and development. Positional cues control what type of cell will form (cell fate determination) and how big the cell will grow (cell expansion). Genetic studies identified membrane-anchored calpain protease DEFECTIVE KERNEL 1 (DEK1) as an essential regulator of plant growth and development. Our hypothesis is that DEK1 evolved both sensory and effector functions to control robust morphogenetic outputs such as cell expansion, asymmetric cell division and cell fate determination in response to local positional cues. The exact mechanism of DEK1 activation and signal transmission is unknown. Another crucial missing piece in our understanding of DEK1-mediated signaling is the identity of DEK1 molecular targets. In this project we aim to validate putative DEK1 substrate(s) and interacting partners, functionally characterize the role of phosphorylation in DEK1 activation and continue our efforts to solve the DEK1 3D structure. The project builds on published and recent unpublished work of the principal investigator and his collaborators. Unraveling the mechanism of DEK1 action will shed light on one of the key principles of developmental control in plants with broad implications for biotechnology and agriculture.
Duration: 1.8.2018 – 30.6.2022