||In the project we will study polyploid origins, phylogenetic relationships and evolutionary history in species complexes from three Brassicaceae genera: Alyssum, Odontarrhena and Cardamine. We aim to generalize the observed patterns and elucidate major evolutionary processes that have driven diversification and speciation in these polyploid species groups. We will also revise controversial species circumscriptions and propose new taxonomic treatments. A combination of multiple methods will be used: multivariate morphometrics, flow cytometric ploidy level screening, and DNA sequencing of different target regions (including a high-throughput sequencing method). We will also develop an online karyological and nomenclatural database of the tribe Cardamineae that will help to stimulate further evolutionary studies in this group. Our studies will contribute to better understanding of plant evolution and to effective conservation of species diversity of the studied genera.