||Dermoloma is insufficiently known genus of basidiomycetes classified in the order Agaricales (Agaricomycotina). More than 30 species are described worldwide, but only few of them are generally accepted. Opinions about morphological delimitation and number of the species are very variable in the current literature. Traditionally, up to eight species are recognised, although more than 30 European species were described. Our preliminary phylogenetic study suggest existence of more than 25 molecular operation units in Europe that probably represent independent species. Aim of the project is to define European and North American Dermoloma species by means of molecular and morphological methods and to assign them correct names. Fungi of the genus Dermoloma are essential part of grassland ecosystems and accordingly this project will deal with studies of environmental soil samples. We will look for suitable metabarcode for identification of species using next generation sequencing (metagenomics).