Aktivácia, regulácia a kontrola bunkových mechanizmov v suspenznej kultúre a intaktných rastlinách tabaku elicitovaných mykotoxínmi húb rodu Fusarium
Activation, regulation and control of cellular mechanisms in suspension culture and intact tobacco plants elicited with mycotoxins of genus Fusarium
Program: VEGA
Zodpovedný riešiteľ: RNDr. Repka Vladimír CSc.
Annotation: In terms of food safety, the focus was primarily on the study of the effects of mycotoxins on the biological activity of the cells of animals. Recently, it is beginning to examine the effect of mycotoxins on the plant, particularly the agronomically interesting. The project aims to physiological, biochemical and molecular analysis of basal cell mechanisms of tobacco plants exposed to different fusariotoxins. We will follow anatomic changes in elicited cells, the role of active oxygen species in the process of perception and pharmacological modulation of the responses to mycotoxins elicitation. We will focus on a comparative analysis of regulatory and signaling cascades involved in the response of cells to various fusariotoxins. We will monitor the expression of genes and proteins, as well as the activity of enzymes responsible for the activation, regulation and control of cell response to mycotoxins, which will bring new insights into understanding the principle mechanisms in respect to biotic stress.
Duration: 1.1.2017 – 31.12.2020
ROLOFSYTS – Funkčná analýza synaptotagmínov so zreteľom na odpovede rastlín na environmentálne stresy
Functional analysis of synaptotagmins in responses of plants to environmental stresses
Program: APVV
Zodpovedný riešiteľ: Doc. RNDr. Jásik Ján CSc.
Annotation: Crop yields are largely dependent on their tolerances to environmental influences, including salinity. Previously, we have shown that the Arabidopsis synaptotagmin 1 (AtSYT1), the most abundantly expressed Arabidopsis ortholog of the well-known calcium sensors in animal neurons, is involved in responses to abiotic stresses. The general goal of this proposal is to reveal the mechanisms by which AtSYTs regulate the reactions to high salinity and other stresses. We wish to characterize AtSYTs expression, describe phenotype of Atsyts mutant lines, to study subcellular localisation of AtSYTs, and to monitor intracellular dynamics of AtSYT1. Additionally, we shall address the functional analysis of the interaction fished out in the yeast two hybrid assay connecting AtSYT1 to the MAPK cascade. Our study will contribute to the overall understanding of how membrane trafficking is involved in stress responses.
Duration: 1.6.2017 – 30.6.2021
Transkripčná, posttranskripčná a posttranslačná regulácia génu IRT1, rozhodujúceho transportéra železa v koreni rastlín
Transcriptional, post-transcriptional and post-translational regulation of IRT1 gene, a key transporter of iron in plant roots
Program: VEGA
Zodpovedný riešiteľ: Doc. RNDr. Jásik Ján CSc.
Annotation: Using transgenic and mutant lines in combination with the current genetic, molecular biological, histochemical, immunological, fluorometric, chemical analytical and microscopic methods we want to explore whether and how the presence or absence of iron and other metal elements transferred by IRT1 protein may affect the activity of IRT1 promoter, the process of RNA splicing, localization, turnover and intracellular dynamics of IRT1 protein varieties in roots of the model organism Arabidopsis thaliana. Examination of multi-level regulation of the IRT1 gene and simultaneous monitoring of the contents and the distribution of iron and other metallic components in the plants will yield valuable information for applications in biotechnology in order to enrich the plants with iron and / or to limit the absorption of potentially toxic metal elements. The findings may be useful in the agricultural breeding practice to obtain higher yielding varieties and tolerant plants to environmental stress caused by heavy metals.
Duration: 1.1.2017 – 31.12.2020