POLYPLOID – Pôvod polyploidných komplexov: úloha polyploidizácie a geografickej a ekologickej izolácie
The origin of polyploid complexes: the role of polyploidization, geographical and ecological isolation
Program: APVV
Zodpovedný riešiteľ: prof. RNDr. Marhold Karol CSc.
Annotation: The main topics of the proposed project are microevolutionary and speciation processes in diploid-polyploid complexes belonging to the Brassicaceae family. Speciation both at the diploid and polyploid levels will be examined, elucidating the role of genome merge and duplication, geographical and ecological separation. The questions addressed have broader relevance, beyond the studied genera, and the answers can help us better understand processes underlying polyploid speciation as such. The methods applied in this project will include both conventional molecular systematics markers, as well as methods based on the Next Generation Sequencing (NGS), namely restriction site associated DNA sequencing (RAD-seq) and combination of target enrichment of low-copy nuclear genes and genome skimming (Hyb-seq). Using the multi-marker approach we expect to gain deeper insights into the studied species complexes and increased robustness of the results. As a complement, flow-cytometric, morphometric, and ecological and climatic niche analyses will be employed. In order to achieve these goals we have chosen four genera from three different tribes of the Brassicaceae family (Alysseae [Alyssum, Odontarrhena], Camelineae [Arabidopsis], Cardamineae [Cardamine]), representing different evolutionary lineages in this family. We will take the advantage of the wealth of methods that are available for and already tested in the closely related genus Arabidopsis, our previous long-term experience with the proposed study genera, and also previously collected plant material.
Duration: 1.8.2018 – 31.7.2022
Prečo sú niektoré druhy úzko endemické a iné široko rozšírené? Štúdium významu biologických vlastností a genetickej variability v troch skupinách blízko príbuzných taxónov z čeľade Asteraceae
Why are some species narrowly endemic while their congeners are geographically widespread? Role of biological traits and genetic variability studied in the three groups of the closely related taxa from the family Asteraceae
Program: VEGA
Zodpovedný riešiteľ: RNDr. Šingliarová Barbora PhD.
Annotation: The project aims at study of determinants of species rarity in thethree model groups from the family Asteraceae (Tephroseris longifolia agg., Cyanus napulifer group and Pilosella alpicola group) by comparing various biological traits and genetic diversity of the rare (often endemic) and widespread congeners. A combined approach including geneticanaly sesemploy in ghighly variable molecular markers (AFLP, SSRs) as well as analyses of fitness, ecological niche and spreading potential (by common garden experiments, GIS digitallayers, soil analyses) will be applied. Results of the project will bring valuable information on specific factors determining range size in selected plant groups from Asteraceae family, but also help to find common features and more general regularities in unravelled patterns and processes. Moreover, outputs of this project will serve as a baseline for conservation recommendations as each of systematic groups investigated harbours also taxa endangered by recent human activities.
Duration: 1.1.2015 – 31.12.2018