||Spatio-temporal changes translate to changes in diversity and distribution of populations and communities across all organizational levels, influencing many aspects of biota. Shifts in distribution of plant communities and species including invasions by alien species gained significance in past decades when this phenomenon became more evident. Now it is obvious that the main drivers and causes of changes in diversity and distribution include 1) land use and management; 2) climate change; 3) spreading of alien species and 4) natural disturbances. Combinations of these factors influence vegetation, agroecosystems and forestry either in negative or positive way. Identifying general pattern at local scales, in various habitats, and differentiation of effects caused by each driver will help us to understand the impact of observed changes and their role in ecosystems. To reach our goals we focus on habitats from the basins to the alpine belt – floodplains, montane forests, grassland and alpine communities.