||Solitary tree standing out of continual forest vegetation has well-known positive and negative impacts on grasslands and arable cultures. Close vicinity of tree causes light and wind interception resulting in change of herbal diversity of grasslands or inhibited growth of crop plants in arable agricultures. Positive influences of tree presence are local enhancing of biological diversity associated with improved ecosystem function. Soil fertility, stability of ecosystem and sustainable land management essentially depends on soil quality. Soil microorganisms are very susceptible to changes in soil characteristics and play the fundamental role in nutrient recycling and stability of ecosystem. Changes in microbial communities connected to presence of trees are underexplored but very actual with current effort to improve landscape structure. Trees are associated with specific groups of fungi; among them are highlighted ectomycorrhizal fungi. Soil of semi-natural grasslands is often dominated by probably biothrophic basidiomycetes and ascomycetes known as CHEGD fungi. In arable soils, special importance is attributed to arbuscular mycorrhizal fungi and presence of parasitic fungi. This project aims to study representation of fungi with symbiotic, saprophytic, parasitic or other trophic guilds resulted from interaction of tree presence in agricultural land. In addition, we will evaluate selected abiotic and biotic soil characteristics to estimate changes in functional diversity. Project uses advances methods in sequencing of environmental DNA, soil analyses and computer modelling of special influence of ecological factors.